Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis.
Identifieur interne : 000061 ( Main/Exploration ); précédent : 000060; suivant : 000062Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis.
Auteurs : Lucero Romero-Aguilar [Mexique] ; Guadalupe Guerra-Sánchez [Mexique] ; Eda P. Tenorio [Mexique] ; Miguel Tapia-Rodriguez [Mexique] ; Genaro Matus-Ortega [Mexique] ; Oscar Flores-Herrera [Mexique] ; James González [Mexique] ; Juan Pablo Pardo [Mexique]Source :
- Archives of microbiology [ 1432-072X ] ; 2020.
Descripteurs français
- KwdFr :
- Antifongiques (pharmacologie), Complexe-1 cible mécanistique de la rapamycine (métabolisme), Lipides (analyse), Métabolisme lipidique (effets des médicaments et des substances chimiques), Sirolimus (pharmacologie), Triglycéride (administration et posologie), Ustilago (composition chimique), Ustilago (effets des médicaments et des substances chimiques), Vacuoles (composition chimique).
- MESH :
- administration et posologie : Triglycéride.
- analyse : Lipides.
- composition chimique : Ustilago, Vacuoles.
- effets des médicaments et des substances chimiques : Métabolisme lipidique, Ustilago.
- métabolisme : Complexe-1 cible mécanistique de la rapamycine.
- pharmacologie : Antifongiques, Sirolimus.
English descriptors
- KwdEn :
- MESH :
- chemical , administration & dosage : Triglycerides.
- chemical , analysis : Lipids.
- chemical , metabolism : Mechanistic Target of Rapamycin Complex 1.
- chemical , pharmacology : Antifungal Agents, Sirolimus.
- chemistry : Ustilago, Vacuoles.
- drug effects : Lipid Metabolism, Ustilago.
Abstract
The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.
DOI: 10.1007/s00203-020-01833-y
PubMed: 32088730
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Universitaria, Coyoacán, 04510, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Guerra Sanchez, Guadalupe" sort="Guerra Sanchez, Guadalupe" uniqKey="Guerra Sanchez G" first="Guadalupe" last="Guerra-Sánchez">Guadalupe Guerra-Sánchez</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio Y Plan de Ayala S/N, Miguel Hidalgo, 11340, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio Y Plan de Ayala S/N, Miguel Hidalgo, 11340, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Tenorio, Eda P" sort="Tenorio, Eda P" uniqKey="Tenorio E" first="Eda P" last="Tenorio">Eda P. 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Universitaria, Coyoacán, 04510, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Matus Ortega, Genaro" sort="Matus Ortega, Genaro" uniqKey="Matus Ortega G" first="Genaro" last="Matus-Ortega">Genaro Matus-Ortega</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Flores Herrera, Oscar" sort="Flores Herrera, Oscar" uniqKey="Flores Herrera O" first="Oscar" last="Flores-Herrera">Oscar Flores-Herrera</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez, James" sort="Gonzalez, James" uniqKey="Gonzalez J" first="James" last="González">James González</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Pardo, Juan Pablo" sort="Pardo, Juan Pablo" uniqKey="Pardo J" first="Juan Pablo" last="Pardo">Juan Pablo Pardo</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico. pardov@bq.unam.mx.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author></analytic><series><title level="j">Archives of microbiology</title><idno type="eISSN">1432-072X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal Agents (pharmacology)</term><term>Lipid Metabolism (drug effects)</term><term>Lipids (analysis)</term><term>Mechanistic Target of Rapamycin Complex 1 (metabolism)</term><term>Sirolimus (pharmacology)</term><term>Triglycerides (administration & dosage)</term><term>Ustilago (chemistry)</term><term>Ustilago (drug effects)</term><term>Vacuoles (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (pharmacologie)</term><term>Complexe-1 cible mécanistique de la rapamycine (métabolisme)</term><term>Lipides (analyse)</term><term>Métabolisme lipidique (effets des médicaments et des substances chimiques)</term><term>Sirolimus (pharmacologie)</term><term>Triglycéride (administration et posologie)</term><term>Ustilago (composition chimique)</term><term>Ustilago (effets des médicaments et des substances chimiques)</term><term>Vacuoles (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Triglycerides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lipids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mechanistic Target of Rapamycin Complex 1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antifungal Agents</term><term>Sirolimus</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Triglycéride</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Lipides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ustilago</term><term>Vacuoles</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Ustilago</term><term>Vacuoles</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lipid Metabolism</term><term>Ustilago</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Métabolisme lipidique</term><term>Ustilago</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Complexe-1 cible mécanistique de la rapamycine</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term><term>Sirolimus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32088730</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-072X</ISSN><JournalIssue CitedMedium="Internet"><Volume>202</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Archives of microbiology</Title><ISOAbbreviation>Arch Microbiol</ISOAbbreviation></Journal><ArticleTitle>Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis.</ArticleTitle><Pagination><MedlinePgn>1211-1221</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00203-020-01833-y</ELocationID><Abstract><AbstractText>The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Romero-Aguilar</LastName><ForeName>Lucero</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guerra-Sánchez</LastName><ForeName>Guadalupe</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio Y Plan de Ayala S/N, Miguel Hidalgo, 11340, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tenorio</LastName><ForeName>Eda P</ForeName><Initials>EP</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tapia-Rodriguez</LastName><ForeName>Miguel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Matus-Ortega</LastName><ForeName>Genaro</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Flores-Herrera</LastName><ForeName>Oscar</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>González</LastName><ForeName>James</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pardo</LastName><ForeName>Juan Pablo</ForeName><Initials>JP</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6108-9705</Identifier><AffiliationInfo><Affiliation>Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico. pardov@bq.unam.mx.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>254904</GrantID><Agency>Consejo Nacional de Ciencia y Tecnología (CONACYT)</Agency><Country></Country></Grant><Grant><GrantID>256520</GrantID><Agency>Consejo Nacional de Ciencia y Tecnología (CONACYT)</Agency><Country></Country></Grant><Grant><GrantID>IPN-SIP-20180625</GrantID><Agency>Instituto Politécnico Nacional-Secretaria de Investigación y Posgrado</Agency><Country></Country></Grant><Grant><GrantID>PAPIIT IN222117</GrantID><Agency>Universidad Nacional Autónoma de México, Programa de Apoyo a Proyectos de Investigación Tecnológica PAPIIT</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Arch Microbiol</MedlineTA><NlmUniqueID>0410427</NlmUniqueID><ISSNLinking>0302-8933</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008055">Lipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014280">Triglycerides</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber><NameOfSubstance UI="D000076222">Mechanistic Target of Rapamycin Complex 1</NameOfSubstance></Chemical><Chemical><RegistryNumber>W36ZG6FT64</RegistryNumber><NameOfSubstance UI="D020123">Sirolimus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050356" MajorTopicYN="N">Lipid Metabolism</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008055" MajorTopicYN="N">Lipids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000076222" MajorTopicYN="N">Mechanistic Target of Rapamycin Complex 1</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014280" MajorTopicYN="N">Triglycerides</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014588" MajorTopicYN="N">Ustilago</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014617" MajorTopicYN="N">Vacuoles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Lipid droplets</Keyword><Keyword MajorTopicYN="N">Rapamycin</Keyword><Keyword MajorTopicYN="N">TOR</Keyword><Keyword MajorTopicYN="N">Ustilago maydis</Keyword><Keyword MajorTopicYN="N">Vacuoles</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32088730</ArticleId><ArticleId IdType="doi">10.1007/s00203-020-01833-y</ArticleId><ArticleId IdType="pii">10.1007/s00203-020-01833-y</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Mexique</li></country></list><tree><country name="Mexique"><noRegion><name sortKey="Romero Aguilar, Lucero" sort="Romero Aguilar, Lucero" uniqKey="Romero Aguilar L" first="Lucero" last="Romero-Aguilar">Lucero Romero-Aguilar</name></noRegion><name sortKey="Flores Herrera, Oscar" sort="Flores Herrera, Oscar" uniqKey="Flores Herrera O" first="Oscar" last="Flores-Herrera">Oscar Flores-Herrera</name><name sortKey="Gonzalez, James" sort="Gonzalez, James" uniqKey="Gonzalez J" first="James" last="González">James González</name><name sortKey="Guerra Sanchez, Guadalupe" sort="Guerra Sanchez, Guadalupe" uniqKey="Guerra Sanchez G" first="Guadalupe" last="Guerra-Sánchez">Guadalupe Guerra-Sánchez</name><name sortKey="Matus Ortega, Genaro" sort="Matus Ortega, Genaro" uniqKey="Matus Ortega G" first="Genaro" last="Matus-Ortega">Genaro Matus-Ortega</name><name sortKey="Pardo, Juan Pablo" sort="Pardo, Juan Pablo" uniqKey="Pardo J" first="Juan Pablo" last="Pardo">Juan Pablo Pardo</name><name sortKey="Tapia Rodriguez, Miguel" sort="Tapia Rodriguez, Miguel" uniqKey="Tapia Rodriguez M" first="Miguel" last="Tapia-Rodriguez">Miguel Tapia-Rodriguez</name><name sortKey="Tenorio, Eda P" sort="Tenorio, Eda P" uniqKey="Tenorio E" first="Eda P" last="Tenorio">Eda P. Tenorio</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32088730
|texte= Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis.
}}
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